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| Registros recuperados: 17 | |
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| Klein, Geraldine; Soum-soutera, Emmanuelle; Bazire, Alexis; Dreanno, Catherine; Compere, Chantal; Dufour, Alain. |
| Bacterial biofilms, frequently in association with algae, protozoa and fungi, are found on all submerged structures in the marine environment. Biofilms are responsible for a range of surface‐associated and diffusible signals, which may moderate the settling behaviour of cells, spores and larvae. Thus, marine microorganisms are a new source of bioactive compounds, which enhance or inhibit the settlement of organisms. |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00089/20005/17667.pdf |
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| Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno. |
| A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-mu L straws or 1.5-mL cryovials. A dilution to 1/3 in Mounib's extender and a cooling rate of 65 degrees C.min(-1) allowed frozen sperm to recover an initial motility similar to that of fresh sperm at thawing; however, significant differences in motility (P < 0.001, n = 10 fish semen) were observed at further post-activation times, the motility decrease being faster in thawed sperm. At the experimental scale, triplicate inseminations of 2-mL aliquots (approximately 2 000 eggs) showed a significant fertility decay of thawed sperm compared to that of fresh sperm (P < 0.01, n = 12 fish semen) when a discriminating 35.10(3) spermatozoa to egg ratio was applied.... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Cryobank; Insemination; Spermatozoa; Cryopreservation; Dicentrarchus labrax; Loup; Banque de sperme; Insémination; Cryoconservation; Cryoconservation. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-866.pdf |
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| Suquet, Marc; Dreanno, Catherine; Fauvel, Christian; Cosson, J; Billard, R. |
| Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evaluated using previously standardized biotests, such as a two-step motility activation technique adapted for the different species and fertilization assays using a discriminating insemination technique. Most extenders used in marine fish are saline or sugar solutions. From the investigated cryoprotectants, dimethyl sulphoxide (DMSO) generally leads to the best results.... |
| Tipo: Text |
Palavras-chave: DMSO; Insemination; Fertilization; Cryopreservation; Fish sperm. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-611.pdf |
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| Sousa, Celia; Compere, Chantal; Dreanno, Catherine; Crassous, Marie-pierre; Gas, Fabienne; Baus, Beatrice; Perrot, Hubert. |
| In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cells concentration. This simple system is potentially promising for environmental monitoring purposes. |
| Tipo: Text |
Palavras-chave: Alexandrium minutum; Quartz microbalance; Microalgae; Immunosensor. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00194/30492/28897.pdf |
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| Lazerges, M.; Perrot, H.; Rabehagasoa, N.; Compere, Chantal; Dreanno, Catherine; Mucio Pedroso, M.; Faria, R. C.; Bueno, P. R.. |
| The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and... |
| Tipo: Text |
Palavras-chave: Biosensors; DNA; Hybridization kinetics; Quartz crystal microbalance. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00101/21195/19415.pdf |
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| Chapelle, Annie; Le Bec, Claude; Amzil, Zouher; Dreanno, Catherine; Guillou, Laure; Klouch, Khadidja; Labry, Claire; Le Gac, Mickael; Pineau-guillou, Lucia; Siano, Raffaele; Abernot, Chantal; Andrieux, Francoise; Caradec, Florian; Destombe, Christophe; Dia, A.; Doner, Anne; Duval, Julia; Gouriou, Jeremie; Lazure, Pascal; Le Brun, Luc; Le Gal, Dominique; Malestroit, Pascale; Petton, Sebastien; Plus, Martin; Quere, Julien; Savar, Veronique; Schmitt, Sophie; Terre, A.; Youenou, Agnes. |
| Suite à l’événement de grande ampleur d’efflorescence d’Alexandrium minutum et des toxicités associées en Rade de Brest en 2012, un projet a été élaboré avec la Région Bretagne dans le cadre du développement du port de Brest. L’Ifremer a organisé un échantillonnage dans l’estuaire de la rivière de Daoulas, zone la plus touchée en 2012, au cours des printemps-été 2013 et 2014. Un suivi de la présence d’A. minutum dans les sédiments a également été réalisé en hiver 2014. De ces suivis, des mesures réalisées au laboratoire à ce jour, des simulations du modèle Prévimer ainsi que des résultats du réseau Rephy et Velyger, il ressort que: * La rade de Brest peut aujourd’hui être considérée comme une zone à risque pour Alexandrium minutum. En effet, depuis 2012... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00254/36487/35029.pdf |
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| Chapelle, Annie; Le Bec, Claude; Le Gac, Mickael; Labry, Claire; Amzil, Zouher; Guillou, Laure; Dreanno, Catherine; Pineau-guillou, Lucia. |
| Suite à l’événement de grande ampleur d’efflorescence d’Alexandrium minutum et des toxicités associées en Rade de Brest en 2012, un projet a été monté avec la Région dans le cadre du développement du port de Brest. De juillet à août 2013, l’Ifremer a organisé un échantillonnage serré dans l’estuaire de Daoulas, zone la plus touchée en 2012. De ce suivi et des mesures réalisées au laboratoire à ce jour, ainsi que des résultats du réseau Rephy et Velyger, il ressort que : * Alexandrium minutum s’est développé à nouveau en rade de Brest en 2013 mais à des teneurs inférieures à 2012. * l’épisode de bloom et de toxicité supérieur aux seuils légaux s’est déroulé plus tard qu’en 2012, du 1er juillet jusqu’à 5 août. * les efflorescences et la toxicité ont touché... |
| Tipo: Text |
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| Ano: 2013 |
URL: https://archimer.ifremer.fr/doc/00191/30230/28644.pdf |
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| Foucher, Eric; Blanchard, Michel; Cugier, Philippe; Desroy, Nicolas; Dreanno, Catherine; Fauchot, Juliette; Fifas, Spyros; Guyader, Olivier; Jean, Frederic; Le Gallic, Bertrand; Riou, Philippe; Schapira, Mathilde; Thiebaut, Eric. |
| The King scallop Pecten maximus constitutes the first landed species in terms of tonnage and the second or third one in terms of value for the French fisheries. More than 90% of these landings come from the English Channel indicating that its exploitation is essential to local fleets. The COMANCHE project (Ecosystem Interactions and anthropogenic impacts on King scallops populations in the English Channel) proposed to improve the knowledge of the scallop within the Channel, through an ecosystem-based approach for fisheries, appealing to a wide range of scientific disciplines (physics, chemistry, genetics, ecology, geostatistics, modeling, economics ....). Researches on the spatial location of scallop beds, connectivity by larval dispersal between beds,... |
| Tipo: Text |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00251/36236/34797.pdf |
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| Suquet, Marc; Dreanno, Catherine; Petton, Bruno; Normant, Yvon; Omnes, Marie-helene; Billard, Roland. |
| The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use... |
| Tipo: Text |
Palavras-chave: Turbot; Psetta maxima; Sperm; Cryopreservation; Hatching. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00176/28721/27176.pdf |
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| Cosson, J; Groison, A; Suquet, Marc; Fauvel, Christian; Dreanno, Catherine; Billard, R. |
| After along period of spermatogenesis (several weeks to months), marine fish spermatozoa are delivered at male spawning in seawater (SW) at the same time as ova. In some fish species, as the ova micropyle closes quickly after release, these minute unicells, the spermatozoa, have to accomplish their task of reaching the micropyle within a very brief period (several seconds to minutes), for delivery of the haploid male genetic information to the ova. To achieve this goal, their high-performance motile equipment, the flagellum, must fully activate immediately on contact with the SW and then propel the sperm cell at an unusually high initial velocity. The cost of such 'hyperactivity' is a very rapid consumption of intracellular ATP that outstrips the supply.... |
| Tipo: Text |
Palavras-chave: Cell activation; Osmolality; Ions; Cell energetics; Motility; Spermatozoon; Marine fish. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6967.pdf |
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| Klein, Geraldine; Soum-soutera, Emmanuelle; Bazire, Alexis; Dreanno, Catherine; Compere, Chantal; Dufour, Alain. |
| Marine organisms represent a rather unexplored source of new activity and biological functions of molecules for biotechnologies. The research in chemical ecology of the marine environment leads to looking at metabolites of recognition or defence produced by these models. Marine bacteria belonging to the Pseudoalteromonas genus of the Gammaproteobacteria class are often found in association with marine eukaryotes, and their ability to produce a variety of biological activities attracted a particular attention. The marine Pseudoalteromonas sp. 3J6 and D41 were selected for their capacity to inhibit the biofilm formation of other bacteria. The study of antibiofilm metabolites synthesised by marine bacteria 3J6 and D41 biofilms can lead to the development of... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00089/19990/17666.pdf |
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| Noyer, Charlotte; Abot, Anne; Trouilh, Lidwine; Leberre, Veronique Anton; Dreanno, Catherine. |
| Detection of harmful algal blooms has become a challenging concern because of to the direct impacts on public health and economy. The identification of toxic dinoflagellates and diatoms in monitoring programs requires an extensive taxonomic expertise and is time consuming. Advances in molecular biology have allowed the development of new approaches, more rapid, accurate and cost-effective for detecting these microorganisms. In this context, we developed a new DNA microarray (called, Phytochip) for the simultaneous detection of multiple HAB species with a particular emphasis on Pseudo-nitzschia species. Oligonucleotide probes were designed along the rRNA operon. After DNA extraction, the target rDNA genes were amplified and labeled using an asymmetric PCR;... |
| Tipo: Text |
Palavras-chave: HAB; Pseudo-nitzschia; Microarray; Ribosomal oligonucleotide probes; Environmental monitoring. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00254/36554/42346.pdf |
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| Colas, Florent; Laurent, Sebastien; Lehaitre, Michel; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Dreanno, Catherine; Deuff, Albert; Compere, Chantal. |
| For the last few years there is a more and more pressing need to develop system for detecting HAB at their early stage. Researchers have then been working on new in situ sensor. In this paper a submarine transducer based on surface plasmon resonance is reported. It demonstrated a refractive index resolution of 3.10(-6), which is promising for later phytoplankton detection. |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00107/21802/20328.pdf |
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| Registros recuperados: 17 | |
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